Here we report the construction of a set of retroviral vectors encoding 23,742 distinct shRNAs, which target 7,914 different human genes for suppression. We and others have recently developed expression vectors to direct the synthesis of short hairpin RNAs (shRNAs) that act as short interfering RNA (siRNA)-like molecules to stably suppress gene expression 4, 5. In mammalian cells, such screens have been hampered by a lack of suitable tools that can be used on a large scale. Please click on the pathway links on the left for additional relevant product information.RNA interference (RNAi) is a powerful new tool with which to perform loss-of-function genetic screens in lower organisms and can greatly facilitate the identification of components of cellular signalling pathways 1, 2, 3. GeneTex offers an extensive catalog of antibody reagents to study key receptors and their associated signaling pathways. In summary, cell signaling defines the very notion of a cell being alive. A common flow for a signaling pathway involves the binding of a ligand to a receptor, protein modification by enzymes like kinases, assembly of signaling complexes and activation of secondary messengers, and ultimately alterations in the actions of transcription factors or other effectors of the transcriptome or proteome. In the most fundamental sense, this allows an extracellular stimulus to exert an effect across the cell membrane through its transduction into a biochemical currency that in most cases will affect gene expression and the cell’s physiological functioning or identity. Cell signaling refers to the various sequences of molecular events that mark a cell’s response to its environment, whether that entails interaction with another cell or other external cues.
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